Cytotoxic Effect of Annona muricata leaf extracts on tumor cell lines in vitro

Abstract

Cancer is a major cause of mortality and morbidity globally and overall survival rate is still low despite advances in surgery, radiotherapy, and chemotherapy. Plants have played a significant role in the treatment of cancer and infectious diseases for a very long time. Annona muricata has been widely used in the treatment of cancer and diseases in many countries. This study was designed to evaluate the cytotoxic effects of Annona muricata ethanolic extracts on (MAD_MB_231) human breast cancer cell lines and lung cancer cell line (A549). Various concentrations of ethanolic extracts (50, 100,150 and 200 µg/mL) were prepared with ethanolic solvents and used to treat cell lines after 24 h. exposure by MTT assay. The results showed the effect of extract after 24- hour treatment is a dose dependent and has the most effective anticancer activity on the lung cells line with IC50 Values of The IC₅₀ was 134.6 mg/ml for breast cancer cell lines and The IC₅₀ was (124.6) mg/ml for lung cancer cell line. the current study showed the highly effective action of the ethanolic crude leaves extract of Annona muricata and can be used in the management and treatment of cancer.