Investigation the phytochemicals constituents, total secondary metabolites contents and anti-oxidant potential of Vitex negundo L.

Abstract

This research aimed to analyze the phytochemical constituents and antioxidant capabilities of Vitex negundo L., which considered as one of medical plant. Leaves were gathered for examination followed by methanolic and essential oil extraction for biological application. Through screening processes important compounds such as flavonoids; which it rich (+++) and the lowest both alkaloids and polyphenols (+) were identified with therapeutic properties attributed to each. A thorough examination revealed an amount of flavonoids (513,34±23,15μg/mL) and complete phenolics (350,67±27,68mg/mL) recognized for their antioxidative properties and contribution, to lowering oxidative pressure.

Antioxidant tests were carried out using DPPN and FRAP methods, for evaluation purposes in the study at hand. In the FRAP experiment conducted on V. negundo L. plant samples showed a ferric reduction capacity with the value of (1.92±30mg/mL) observed at concentrations compared to the standard Trolox which was recorded as (0.545±15mg/mL). For the DPPN test results, on V. negundo L. indicated a substantial ability to scavenge radicals where it achieved a percentage of (83.34±3.51μg/mL) concentration level surpassing even Vitamin C which is known for its antioxidant properties. These significant findings highlight V. negundo L. potential as a natural source of antioxidants.

These results indicate that V. negundo L. may be an option, for nutraceutical purposes in addressing conditions linked to oxidative stress. This supports its standing use and positions it as a remedy, in contemporary medicine.