Genetic variations of rad51 gene in Iraqi breast cancer patients

Abstract

In a group of women with early-stage breast cancer in Baghdad/Iraq, this study has been done to assess the impact of certain SNPs situated on RAD51 genes on its repair effects on DNA damage. Between November 2020 and March 2021, (100) FFPE samples from breast cancer patients who were diagnosed in the early stages of the disease were obtained at the Tumor Teaching Hospital in the Medical City and the Al-Amal National Hospital in Baghdad. As a control group, (50) samples from healthy women were also obtained. All of the study samples' DNA was taken in order to find SNPs, and their ages varied from (40 to) 60 years. Additionally, DNA sequence analysis was used to identify single nucleotide polymorphisms (SNPs) in exon 6 of the RAD51 gene. Then, these exons' nucleotide sequences were matched with NCBI and the control group (healthy women). In exon 6 of the RAD51 gene, one polymorphism, rs121917739 GA, was discovered.