Streptomyces actinomycinicus CMU-RKDM30 Bacterial Active Compounds Revealed by High-Performance Liquid Chromatography

Abstract

It is estimated that over 70% of naturally derived antibiotics currently in clinical use are sourced from soil actinomycetes. Some well-known examples of antibiotics produced by actinomycetes include streptomycin, erythromycin, vancomycin, and tetracycline. Streptomyces actinomycinicus is a bacterium species from the genus of Streptomyces which has been isolated from soil. Streptomyces actinomycinicus has the ability to degrade poly(3-hydroxyalkanoate). This species produces Actinomycin, exfoliamycin and many secondary metabolite. The 52 isolated obtained from the isolation processes were sub-cultured on yeast extract-malt extract agar. The results obtained of our isolation Streptomyces  actinomycinicus CMU-RKDM30 which detected by PCR based on the study of the 16S rRNA gene sequence and phylogenetic relationship.The results of biochemical tests revealed the amylase, urease, catalase, gelatinase, protease, cellulose, phosphatase tests were positive, while the indole production and soluble pigment tests were negative. The HPLC of extracellular crude extract of our isolation Streptomyces  actinomycinicus showed three different antibiotics (Azithromycin, Amoxicillin, and actinomycin).